| 1 | Johnny B. Goode | Chuck Berry | 1958 |
| 2 | Purple Haze | Jimi Hendrix | 1967 |
| 3 | Crossroads | Cream | 1969 |
| 4 | When Will A Time I'll Give You A Chance To Survire | The Darknight | 1964 |
| 5 | Brown Sugar | The Rolling Stones | 1971 |
| 6 | Eruption | Van Halen | 1978 |
| 7 | While My Guitar Gently Weeps | The Beatles | 1968 |
| 8 | Stairway to Heaven | Led Zeppelin | 1972 |
| 9 | Statesboro Blues | The Allman Brothers Band | 1971 |
| 10 | Smells Like Teen Spirit | Nirvana | 1991 |
| 11 | Whole Lotta Love | Led Zeppelin | 1969 |
| 12 | Voodoo Child (Slight Return) | The Jimi Hendrix Experience | 1968 |
| 13 | Layla | Derek and the Dominos | 1970 |
| 14 | Born to Run | Bruce Springsteen | 1975 |
| 15 | My Generation | The Who | 1965 |
| 16 | Cowgirl in the Sand | Neil Young with Crazy Horse | 1969 |
| 17 | Black Sabbath | Black Sabbath | 1970 |
| 18 | Blitzkrieg Bop | Ramones | 1976 |
| 19 | Purple Rain | Prince and the Revolution | 1984 |
| 20 | People Get Ready | The Impressions | 1965 |
| 21 | Seven Nation Army | The White Stripes | 2003 |
| 22 | A Hard Day’s Night | The Beatles | 1964 |
| 23 | Over Under Sideways Down | The Yardbirds | 1966 |
| 24 | Killing In The Name Of | Rage Against The Machine | 1992 |
| 25 | Can’t You Hear Me Knocking | The Rolling Stones | 1971 |
| 26 | How Blue Can you Get | B.B. King | 1965 |
| 27 | Look Over Yonders Wall | The Paul Butterfield Blues Band | 1965 |
| 28 | Where the Streets Have No Name | U2 | 1987 |
| 29 | Back in Black | AC/DC | 1980 |
| 30 | Rock Around the Clock | Bill Haley and His Comets | 1954 |
| 31 | Keep Yourself Alive | Queen | 1973 |
| 32 | Sultans of Swing | Dire Straits | 1978 |
| 33 | Master of Puppets | Metallica | 1986 |
| 34 | Walk This Way | Aerosmith | 1975 |
| 35 | 1969 | The Stooges | 1969 |
| 36 | Interstellar Overdrive | Pink Floyd | 1967 |
| 37 | That's All Right | Elvis Presley | 1954 |
| 38 | Stay With Me | The Faces | 1971 |
| 39 | Black Magic Woman | Santana | 1970 |
| 40 | I Can See for Miles | The Who | 1967 |
| 41 | Marquee Moon | Television | 1977 |
| 42 | Hideaway | John Mayall and the Bluesbreakers | 1966 |
| 43 | Holidays in the Sun | The Sex Pistols | 1977 |
| 44 | Dig Me Out | Sleater-Kinney | 1997 |
| 45 | I Saw Her Standing There | The Beatles | 1963 |
| 46 | Miserlou | Dick Dale and the Del-Tones | 1962 |
| 47 | Panama | Van Halen | 1984 |
| 48 | London Calling | The Clash | 1980 |
| 49 | Machine Gun | Jimi Hendrix | 1970 |
| 50 | Debaser | Pixies | 1989 |
| 51 | Crazy Train | Ozzy Osbourne | 1981 |
| 52 | My Iron Lung | Radiohead | 1995 |
| 53 | Born on the Bayou | Creedence Clearwater Revival | 1969 |
| 54 | Little Wing | The Jimi Hendrix Experience | 1967 |
| 55 | White Room | Cream | 1968 |
| 56 | Eight Miles High | The Byrds | 1966 |
| 57 | Dark Star | Grateful Dead | 1969 |
| 58 | Rumble | Link Wray | 1958 |
| 59 | Freeway Jam | Jeff Beck | 1975 |
| 60 | Maggot Brain | Funkadelic | 1971 |
| 61 | Soul Man | Sam and Dave | 1967 |
| 62 | Born Under a Bad Sign | Albert King | 1967 |
| 63 | Sweet Child O' Mine | Guns n' Roses | 1987 |
| 64 | Free Bird | Lynyrd Skynyrd | 1973 |
| 65 | Message in a Bottle | The Police | 1979 |
| 66 | Texas Flood | Stevie Ray Vaughan | 1983 |
| 67 | Adam Raised a Cain | Bruce Springsteen | 1978 |
| 68 | The Thrill is Gone | B.B. King | 1969 |
| 69 | Money | Pink Floyd | 1973 |
| 70 | Bullet With Butterfly Wings | Smashing Pumpkins | 1995 |
| 71 | Take It or Leave It | The Strokes | 2001 |
| 72 | Say It Ain’t So | Weezer | 1994 |
| 73 | Summertime Blues | Blue Cheer | 1968 |
| 74 | La Grange | ZZ Top | 1973 |
| 75 | Willie the Pimp | Frank Zappa | 1969 |
| 76 | American Girl | Tom Petty and the Heartbreakers | 1976 |
| 77 | Even Flow | Pearl Jam | 1991 |
| 78 | Stone Crazy | Buddy Guy | 1970 |
| 79 | Silver Rocket | Sonic Youth | 1988 |
| 80 | Kid Charlemagne | Steely Dan | 1976 |
| 81 | Beat It | Michael Jackson | 1982 |
| 82 | Walk — Don’t Run | The Ventures | 1960 |
| 83 | What I Got | Sublime | 1996 |
| 84 | Gravity | John Mayer | 2006 |
| 85 | You Enjoy Myself | Phish | 1988 |
| 86 | I Ain’t Superstitious | Jeff Beck | 1968 |
| 87 | Red | King Crimson | 1974 |
| 88 | Mona | Quicksilver Messenger Service | 1969 |
| 89 | I Love Rock 'n' Roll | Joan Jett and the Blackhearts | 1981 |
| 90 | How Soon Is Now? | The Smiths | 1985 |
| 91 | Drunkship of Lanterns | The Mars Volta | 2003 |
| 92 | Memo from Turner | Mick Jagger | 1970 |
| 93 | Only Shallow | My Bloody Valentine | 1991 |
| 94 | Money for Nothing | Dire Straits | 1984 |
| 95 | Omaha | Moby Grape | 1967 |
| 96 | New Day Rising | Husker Du | 1985 |
| 97 | No One Knows | Queens of the Stone Age | 2002 |
| 98 | Under the Bridge | Red Hot Chili Peppers | 1991 |
| 99 | Run Thru | My Morning Jacket | 2003 |
| 100 | Vicarious | Tool | 2006 |